ABSTRACT
Plausible interactions between food contaminants and natural constituents in vivo and protective effect of polyphenols present in I. aquatica against carbofuran toxicity in Charles Foster rats were evaluated. Determinations based on antioxidant enzyme activities showed significant alterations in glutathione, glutathione peroxidase, superoxide dismutase and catalase in tissues (liver and brain) and plasma of pesticide treated group while polyphenolic extracts from I. aquatica (IAE) attenuated their activities when given alongwith carbofuran. IAE decreased enhanced lipid peroxidation levels in plasma and erythrocyte membrane and cholesterol levels in brain and plasma. IAE also minimized histopathological degenerative changes produced by carbofuran. While single cell gel electrophoresis showed that secondary metabolites in leafy vegetables produced a combinatorial effect with pesticide at cellular level, DNA fragmentation level in bone marrow cells showed a decline in the IAE treated rats. Food safety adversely affected by various chemical contaminants can be retained by plant polyphenols and secondary plant constituents that can be found together in bolus. Therefore, the present study gives an insight into the protective role of naturally found polyphenols against pesticide toxicity.
Subject(s)
Animals , Antioxidants/administration & dosage , Antioxidants/chemistry , Carbofuran/toxicity , Catalase/blood , Chemical and Drug Induced Liver Injury/blood , Chemical and Drug Induced Liver Injury/drug therapy , Chemical and Drug Induced Liver Injury/enzymology , Glutathione Peroxidase/blood , Ipomoea/chemistry , Lipid Peroxidation/drug effects , Liver/drug effects , Liver/enzymology , Male , Oxidative Stress/drug effects , Plant Extracts/administration & dosage , Plant Extracts/chemistry , Polyphenols/administration & dosage , Polyphenols/chemistry , Rats , Superoxide Dismutase/bloodABSTRACT
The aim of this study was to evaluate the effect of an organic extract obtained from Ipomoea alba L. (Convolvulaceae or OE 1493), on experimental periodontal disease in rats. Periodontitis was induced in thirty six Wistar rats: a first mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Animals were randomly assigned to two groups and treated topically, three times a day, for 11 days, as follows: Control Group - vehicle-treated (n = 18), and Test Group - OE 1493-treated (n = 18). The rats were sacrificed on the 12th day. Morphometrical measurements from the cementoenamel junction to the bone crest were performed to determine alveolar bone loss, using standardized photographs. Single- and multi-dose acute toxicity assays were carried out after OE 1493 treatment. Morphometrical analysis demonstrated that topically-administered OE 1493 showed no effect on reducing bone loss when compared with the control group (p > 0.05). In addition, OE 1493 did not present toxicity. Within the limits of this investigation, it may be concluded that OE 1493 did not show any positive influence on the progression of ligature-induced periodontitis in rats, when administered according to the regimen used in the present study.
Subject(s)
Animals , Male , Rats , Ipomoea/chemistry , Phytotherapy , Periodontitis/drug therapy , Plant Extracts/therapeutic use , Alveolar Bone Loss/drug therapy , Disease Models, Animal , Disease Progression , Random Allocation , Rats, Wistar , Time FactorsABSTRACT
Methanolic extracts of different parts of five medicinal plants, Ferula assafoetidaL. resin, Grewia asiaticaL. leaves, Ipomoea hederaceaJacq. seeds, Lepidium sativumL. seeds and Terminalia chebulaRetz. fruits were tested in vitrofor their cytotoxic, phytotoxic, insecticidal, nematicidal and anthelmintic activities. Ipomoea hederaceashowed very significant phytotoxic and cytotoxic activity, with 100% inhibition of Lemna minorgrowth and 100% death of Artemia salinaat concentrations of 1000 and 100 µg mL-1. Grewia asiaticaexhibited very weak activities while Lepidium sativumand Ferula assafoetidashowed moderate to good potential in all three bioassays. The results suggest screening of Ipomoea hederaceaseeds further for isolation of bioactive compounds that may be responsible for its toxic potential.
Subject(s)
Animals , Plant Extracts/pharmacology , Ferula/chemistry , Grewia/chemistry , Ipomoea/chemistry , Lepidium sativum/chemistry , Pakistan , Plant Extracts/classification , Terminalia/chemistryABSTRACT
In this study, the radical scavenging capacity of Guiera senegalensis, Ipomoea asarifolia and Anisopus mannii were compared. The ascorbic acid equivalent of the stem bark (2.1 mM), leaves (2.05 mM) and root barks (1.89 mM) of Guiera senegalesis are much higher than any part of Ipomoea asarifolia or Anisopus manii. The stem bark, leaves and root bark of Guiera Senegalensis had IC50 of 15.4, 20.0 and 17.0 µL respectively, while corresponding parts of Ipomoea asarifolia showed IC50 of 50, 42 and 65 µL, respectively. These results suggest that Guiera senegalensis and Ipomoea asarifolia possess significant antioxidant capacities to warrant further detailed studies on the role of this property in their therapeutic effects.
Comparou-se a capacidade de sequestrar radicais livres de Guiera senegalensis, Ipomoea asarifolia e Anisopus mannii. O equivalente em ácido ascórbico da casca do caule (2,1 mM), folhas (2,05 mM) e da casca da raiz (1,89 mM) de Guiera senegalesis foi muito mais alto do que qualquer parte da Ipomoea asarifolia e da Anisopus manii. A casca do caule, as folhas e a casca da raiz de Guiera senegalensis apresentaram IC50 de 15,4, 20,0 and 17,0 µLl, respectivamente, enquanto as partes correspondentes de Ipomoea asarifolia apresentaram IC50 de 50, 42 e 65 µL respectivamente. Esses resultados sugerem que Guiera senegalensis e Ipomoea asarifolia possuem capacidade oxidante significativa que demanda estudos posteriores detalhados acerca do papel dessa propriedade em seus efeitos terapêuticos.
Subject(s)
Apocynaceae/chemistry , Combretaceae/chemistry , Ipomoea/chemistry , Physiology, Comparative , Antioxidants/analysis , Plant Extracts/analysisABSTRACT
Ipomoea imperati (Convolvulaceae) lives on the sandy shores of the Brazilian coast and in other areas of the world. The anti-inflammatory activity of a methanol-water extract of the leaves of I. imperati was investigated in experimental models of acute and subchronic inflammation. Topical application of the extract (10 mg/ear) inhibited mouse ear edema induced by croton oil (89.0 ± 1.3 percent by the lipid fraction with an IC50 of 3.97 mg/ear and 57.0 ± 1.3 percent by the aqueous fraction with an IC50 of 3.5 mg/ear) and arachidonic acid (42.0 ± 2.0 percent with an IC50 of 4.98 mg/ear and 31.0 ± 2.0 percent with an IC50 of 4.72 mg/ear). Phospholipase A2, purified from Apis mellifera bee venom, was also inhibited by the extract (5.0 mg/ml lipid and aqueous fraction) in vitro in a dose-dependent manner (85 percent by the lipid fraction with an IC50 of 3.22 mg/ml and 25 percent by the aqueous fraction with an IC50 of 3.43 mg/ml). The methanol-water extract of I. imperati (1000 mg/kg) administered by the oral route also inhibited the formation of cotton pellet-induced granulomas (73.2 ± 1.2 percent by the lipid fraction and 56.14 ± 2.7 percent by the aqueous fraction) and did not cause gastric mucosal lesions. I. imperati extracts (10 mg/ml) also inhibited in a dose-dependent manner the muscle contractions of guinea pig ileum induced by acetylcholine and histamine (IC50 of 1.60 mg/ml for the lipid fraction and 4.12 mg/ml for the aqueous fraction). These results suggest the use of I. imperati as an anti-inflammatory and antispasmodic agent in traditional medicine